Population dynamics of teak defoliator, Hyblaea puera Cram. (Lep., Hyblaeidae) in teak plantations of Bangladesh, Journal of Applied Entomology, Volume Abstract. Hyblaea puera Cramer (Lepidoptera: Hyblaeidae) was reported in commercial teak (Tectona grandis L. f.) plantations in the states of Campeche and. Hyblaea puera Cramer (Lepidoptera: Hyblaeidae) was reported in commercial teak (Tectona grandis L. f.) plantations in the states of.
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Mitochondrial DNA sequences are frequently transferred to the nucleus-giving rise to NUMTs, which are considered to be common in eukaryotes [ 22 ]. Carabidae of differing habitat preference and flight-wing development.
The molecular weight of each band was estimated by comparing with a co-migrating bp ladder Amersham. Primers were initially screened for polymorphism and repeatability. Authors’ contributions CN and MB performed the molecular studies and are responsible for the interpretation of molecular data whilst TV and VVS performed the field data collection and are responsible for spatial and temporal data interpretation. On Avicennia plants the caterpillars pupate inside the cases made out of the host plant leaves itself.
In contrast, the endemic populations are genetically distant from the epicenter populations.
The NPVs come under the family of baculoviridae and its virions are enveloped rod shaped nucleocapsids containing circular, supercoiled, double stranded DNA. The maximum number of monomorphic bands was observed using marker CB-N Larvae hatch in about two days. Views Read Edit Jyblaea history.
Intl J Ecol Environ Sci. Daly JC, Gregg P. Here it has been effectively used to discriminate the various population groups of H. Five endemic populations, twenty six epicenter populations and seven epidemic populations for the year were included in the study.
It can also be used to discriminate taxonomically various closely — related moths to the species level. Puerq this study, we used a variant of the RAPD approach involving various nuclear and mitochondrial gene specific primers to trace the origin of teak defoliator outbreaks.
This page was last edited on 4 Aprilat This suggests that under a single demographic structure, two phenotypic classes of H.
Each reaction consisted of 1x Taq buffer with 1. It is difficult to predict the exact time and place of these outbreaks.
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Normalization helped us to control the brightness and streakiness of bands without altering the lighter bands and also control the inter-gel mobility shifts.
Evidence from the study of the population dynamics of H. The nuclear and mitochondrial gene specific primers chosen did not produce any amplification product when used in combination with the corresponding primers as described in the UBC primer set kit [ 17 ].
They are usually caught for consumption at the cocoon stage, at which point they are easily collected from the ground. The attack can kill smaller seedlings but usually only decreases the growth increment.
Moth Photographers Group – Hyblaea puera –
In each marker, the average number of bands scored varied from 7— Genetic variation in Heliothis in Australia: Host-based genotype variation in insects revisited. Results The nuclear and mitochondrial nyblaea specific primers chosen did not produce any amplification product when used in combination with the corresponding primers as described in the UBC primer set kit [ 17 ].
Early events in the outbreak of teak caterpillar Hyblaea puera. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Such ;uera have also been used to study founder events [ 5 ], geographical invasions [ 6 ], small and large scale displacements [ 78 ], including movement of entire population demes [ 9 ], and even altitudinal movements related to habitat patchiness and persistence [ 10 ].
Hyblaea puera – Wikipedia
University of York, UK; Ten 5th instar larvae were preserved for DNA isolation from each sample site, whilst the remaining larvae were reared into the next generation. Pests and Diseases of Forest Plantation Trees.
Molecular data can yield valuable information when integrated with information from ethology, field ecology, comparative morphology, systematics and palaeontology [ 11 ]. Author information Article notes Copyright and License information Disclaimer.
Starting with the third instar, the larva cuts out a leaf flap, usually at the edge of the leaf, folds it over, fastens it with silk, and feeds from within. Eggs are laid on tender new leaves, placed singly near the veins, and usually on the undersurface. The larval samples that were geographically close and had a difference of one complete life cycle stage between the population groups were subjected to molecular studies to evaluate their relatedness.
Gene flow, phylogeography and their uses. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Conclusions The present results appear to validate the hypothesis, that control of H.