Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . Un protoplasto es una cĂ©lula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Index terms: Citrus sp., gamma irradiation, grapefruit, protoplast fusion, somatic foi de protoplastos de ‘Ruby Red’ irradiados com 80 Gy, fusionados com.

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Cytological and RAPD marker analysis confirm their hybrid nature. A slot-blot hybridization method for screening somatic hybrids.

Both 6carboxyfluorescein and the kanamycin fuin harboured by the plasmid have been transferred from liposomes to protoplasts of phycomyces by the fusion technique. Final concentration was protoplasts per millilitre. Protoplasts fusion from S. Under these conditions, calli hybrids protoplastis the developmental pathway of the morphogenic parents. This technique is a tool for somatic hybrid characterization Baird et al. Then the suspension was centrifugated at rpm during ten minutes.

Fusion de Protoplastos by Arely Gutierrez on Prezi

Somatic hybridization protoplzstos the genus Solanum: Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.


Molecular genetic characterization of plant somatic hybrids.

Abstract Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. Analysis of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and Solanum sanctae-rosae.

Shoots also showed purple-colored meristematic zones.

Protoplast fusion, culture and regeneration Prior to fusion, protoplasts from S. Heterokaryons can not be identified because protoplasts of the parents were of the same type and shape. We describe here fusion between phospholipid vesicles liposomes and protoplasts to the fungus phycomyces blakesleeanus. After seven days, the culture medium was diluted 1: The morphology of fusion-derived regenerants was intermediate between the donor parents.

In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S.

Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. Plant Cell Tissue Organ Cult. After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur.

Fusion de protoplastos pdf

These results showed that all hybrids were aneuploids Fig. Interspecific somatic hybrids between wild potato Solanum acaule Bitt. Protoplasts fusion and plant regeneration. Genetic Resourses Conservation and Characterization Division.


Nineteen plants were regenerated from calli Fig. Successful application of the fusion technique demands a protocol for plant regeneration from protoplasts. Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. Protoplasts were precipitated by centrifugation at rpm during five minutes.

Cultivo y fusion de protoplastos

This can be done via morphological, biochemical, cytological and molecular markers Pinto et al. This value is different from that obtained by Mattheij et al. Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange.

Root tips were stained by lacto-propionic orcein.

Microcalli were transferred onto MS medium to induce shoot regeneration under indirect light conditions.