El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Under these conditions, calli hybrids follow the protopllastos pathway of the morphogenic parents. Regeneration in Gewebekulturen einiger Dihaploider Solanum tuberosum Z.

This technique has created novel cellular genome configurations by combining sexually incongruent species. The best study guides.

Cultivo de protoplastos de yuca y Stylosanthes

What students say about Stuvia. Identification of somatic hybrids of dihaploid S. This study shows re somatic hybrid potato plants can be obtained by the fusion method presented.

These samples were obtained from rooted plants maintained in a greenhouse. When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking. Earn hundreds of dollars each month by selling your written material to your fellow students. Heterokaryons can not be identified because protoplasts of the parents were of the same type and shape. Plant Cell Tissue Organ Cult.

Fusion-treated protoplasts were cultured in V-KM medium supplemented with bovine serum albumin. Protoplasts fusion from S.


Isolation of plant DNA from fresh tissue. Genetic Resourses Conservation and Characterization Division.

In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S. Potato, Solanum tuberosum L.

Somatoclonal variation in potato: Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L. Nineteen cultigo hybrid plants were obtained from fusion experiments using PEG in an effort to combine elite traits from both parents.

With the study guides and notes written by fellow students, you are guaranteed to be properly prepared for your exams. Everything you need to know about selling on Stuvia. The origin of this chromosome instability has been studied elsewhere in potato Kumar, The wild species S.

None of the plants had a similar banding pattern in relation protollastos each one of the parents. Literature cited Austin S. The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article. Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L. Chromosome observations indicated that all hybrids were aneuploids.

Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei protoplastoos a recombination in protoplasto plastome. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. Production of somatic hybrids by electrofusion in Solanum.

Regeneration of isolated protoplasts to plants in Solanum dulcamara. Prior to fusion, protoplasts from S. Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. After seven days, the culture medium was diluted 1: Solanum, protoplast fusion, polyethylene glycol, somatic hybrid, RAPD analysis.


What ed you want to do? Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers.

The numbers of chromosome of S. Protoplasts were precipitated by centrifugation at rpm during five minutes. Regeneration of fertile plants from Helianthus nuttalli and Helianthus giganteus L. Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis.

In this study, confirmation of fusion-derived regenerants by the use of RAPD markers represented an effective system for the detection of somatic hybrids from S. Your fellow students know exactly where the pitfalls lie and what the key elements will be to your success in that module.

Aggregations of more than two protoplasts were also observed.

Cultivo y fusion de protoplastos [1990]

The ploidy of the genotype used in plant regeneration has been shown to profoundly affect the type of variation observed in morphological characters and chromosome numbers among regenerant plants.

Root tips were stained by lacto-propionic orcein. Instpp The RAPD analysis was carried out for all regenerated plants derived from calli of the combination S.

For this analysis, DNA extraction was performed according to Doyle and Doyle with some modifications. A revised medium for rapid growth bioassays with tobacco tissue culture. Analysis of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and Solanum sanctae-rosae.

Universidad Nacional Agraria La Molina.