ASTM E2149 PDF

ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.

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This dynamic shake flash test was developed for routine quality control and screening test.

Facebook Linkedin Instagram Twitter Google. The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions. The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension.

Note 1 — Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation. Test Package Request Form. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.

Work Item s – proposed revisions of this standard. Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.

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Record and report presence of solution activity. Link to Active This link will always route to the current Active version of the standard. The presence of a leaching antimicrobial is determined post-test. Organisms typically used in this method: Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials. Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors.

No other units of measurement are included in this standard. The flask is placed onto a wrist-action shaker and shaken for a desired exposure time, typically 1 hour.

The number of atsm organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. E219 method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims. Log in Log out Edit. This standard does not purport to address all of the safety concerns, if any, associated with its use. In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.

ASTM E – Microbe Investigations (MIS)

This test method is designed to evaluate the resistance of specimens treated with a non-leaching antimicrobial agent to the growth of microbes under dynamic contact conditions.

It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period.

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This website stores cookies on your computer. It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials. Summary of Test Method: Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously. To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required.

The test quantitatively evaluates the effectiveness of a sample treated with a non-leaching antimicrobial agent by shaking in an organism suspension. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.

This standard does not purport to address all of the safety concerns, if any, associated with its use.

ASTM E2149 Antimicrobial Activity

Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent. Link to Active This link will always route to the current Active version of the standard. It is w2149 responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Immobilized antimicrobial agents, such as surface bonded materials, are not free to diffuse into their environment under normal conditions of use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.