40818 DATASHEET PDF

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Phospho-STING (Ser366) (D8K6H) Rabbit mAb #40818

More about how we get our images. Sample Analysis Proceed to one of the following specific set of steps. Discard supernatant in appropriate waste container. Would you like to visit your country specific website? Volumes are for 10 fatasheet x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly. Block specimen in Blocking Buffer for 60 min.

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This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody. Remove buffer once solution is clear. Incubate for at least 5 min at room temperature. Vortex, then microcentrifuge for 30 sec.

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Rinse three datawheet in 1X PBS for 5 min each. Scrape cells off the plate and transfer to microcentrifuge tubes. Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies: While blocking, prepare primary antibody by diluting as indicated on datasheet in Antibody Dilution Buffer. Detection of Proteins Directions for Use: Find answers on our FAQs page.

Briefly vortex the stock tube to resuspend the magnetic beads. Pellet beads using magnetic separation rack. Treat cells by adding fresh media containing regulator for desired time.

Sonicate for 10—15 sec to complete datahseet lysis and shear DNA to reduce sample viscosity. Proceed with detection Section D. To Purchase S View sizes. Carefully remove the buffer once datashedt solution is clear.

Dilute to 1X with dH 2 O. Place the datqsheet in a magnetic separation rack for seconds. USP8 Antibody – Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Protein Blotting A general protocol for sample preparation. Mizuno E et al. Immunoprecipitation for Native Proteins This protocol is intended for immunoprecipitation of native proteins for analysis by western immunoblot or kinase activity utilizing Protein A magnetic separation.

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Proceed to immunoprecipitation section.

Electrotransfer to nitrocellulose membrane Separate the beads from the lysate using a magnetic separation rack, transfer the pre-cleared lysate to a clean tube, and discard the magnetic bead datashheet.

Aspirate fixative, rinse three times in 1X PBS for 5 min each. To harvest cells under nondenaturing conditions, remove media and rinse cells once with ice-cold 1X PBS. Wash cells by centrifugation in excess 1X PBS to remove methanol.

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Incubate with rotation for 20 min at room temperature. Application Dilutions Western Blotting 1: Ubiquitinating enzymes UBEs catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme DUB action 1,2.

Changing to another country might result in loss of shopping cart. To prepare 10 ml, add 0.